Cholesterol synthesis and import contribute to protective cholesterol ... . PDF Targeting CRABP-II overcomes pancreatic cancer drug resistance by ... 2.10. Differences to cholesterol are indicated in green and red; i.e., an extra methyl group and double bond in the alkyl side chain and two additional double bonds . Preparation and flow cytometric analysis of BODIPY-cholesterol labelled boar spermatozoa. Upon injection into the yolk sac, BODIPY-cholesterol did not disturb zebrafish development and was targeted to sterol-enriched brain regions in live fish. Experiments to study LDL are typically performed by adding fluorescently labeled LDL to cultured cells and analyzing . Invitrogen BODIPY 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a ... For the BODIPY-cholesterol assay, ram spermatozoa were labelled with BODIPY-cholesterol and prepared for in vitro capacitation as previously described (Bernecic et al. ) synthesized a cholesterol-Bodipy (Bdp-Chol) derivative (Fig. . Briefly, following dilution to 250 × 10 6 sperm/mL with TALP devoid of BSA and bicarbonate, spermatozoa were labelled with 1 µM BODIPY-cholesterol (1 mM stock solution . Cholesterol, an essential component of membranes, regulates membrane fluidity and thus affects various receptor-mediated signal transduction pathways. Analysis of sterol distribution and transport in living cells has been hampered by the lack of bright, photostable fluorescent sterol derivatives that closely resemble cholesterol. The green signal represents Bodipy; the Hoechst nuclear staining is in blue. 2019). 2 bodipy-cholesterol … BODIPY-cholesterolefflux assaywasperformedas described in aprevious study[20].Briefly, macrophages culturedin24-wellplates(1.5X105 cells/well)werelabeled withBODIPY cho-lesterollabelingmedium for1hourandwashedwithRPMI. (A) Ribbon representation of the crystal structure of monomeric water-soluble PFO shows locations of E167 in D1 and A215 in D3 that were substituted with Cys and labeled with BODIPY.A cross-bar indicates locations of the two residues that formed an intramolecular disulfide bond after substitution by Cys; a star indicates the location of the F318A mutation. 干货满满!荧光染料大总结! - Sohu *P <. Lipids were solubilized at 65 °C in phos-phate-buffered saline by sonication (at 25% of full power setting) with a The absorption and emission bands of DiI-C 12 (in DMSO) are centered at 551 nm and 659 nm, respectively (Fig. BODIPY 493/503 Staining of Neutral Lipid Droplets for ... - PubMed

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